Stable genetic modification of human embryonic stem cells by lentiviral vectors
نویسندگان
چکیده
منابع مشابه
Genetic modification of human embryonic stem cells.
Abbreviations: Ad: adenovirus, bFGF: basic fibroblast growth factor, BMP: bone morphogenetic protein, CNS: central nervous system, ES: embryonic stem, FACS: fluorescence activated cell sorting, HIV: human immunodeficiency virus, ICM: inner cell mass, LIF: leukemia inhibitory factor, MEF: mouse embryonic fibroblast, RNAi: RNA interference, SCNT: somatic cell nucleus transfer, UTR: untranslated r...
متن کاملReprogramming by cytosolic extract of human embryonic stem cells improves dopaminergic differentiation potential of human adipose tissue-derived stem cells
The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...
متن کاملTransduction of human embryonic stem cells by ecotropic retroviral vectors
The steadily increasing availability of human embryonic stem (hES) cell lines has created strong interest in applying available tools for gene transfer in murine cells to human systems. Here we present a method for the transduction of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a construct carrying the murine retrovirus receptor mCAT1. Subsequently, ...
متن کاملTransgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos.
The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells is invaluable for the analysis of gene function and regulation in the living animal. The use of vectors derived from retroviruses as gene transfer vehicles in this setting has had limited success because of silencing of transgene expression. Here, we show that vectors derived from lentiviruses, which are co...
متن کاملGenome modification in human embryonic stem cells.
Induced pluripotent stem cell (iPSC) technology has emerged as the most promising method for generating patient-specific human embryonic stem (ES) cells and adult stem cells (Takahashi et al., 2007, Cell 131:861-872; Wernig et al., 2007, Nature 448:318-324; Park et al., 2008, Nature 451:141-146). So far, most studies of direct reprogramming have been done by using lentiviruses/retroviruses enco...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2003
ISSN: 1525-0016
DOI: 10.1016/s1525-0016(02)00047-3